This week was pretty mellow. All we did was work on our methods presentation and present them. We had Monday and Tuesday to complete the presentation and started presentations on Wednesday. The presentation was only 10 slides plus citations and you only had 6-8 minutes to present. I had trouble keeping my slides condensed because there was so much I needed to add to my presentation. I had three separate slides for the methods and materials needed for each phase of my experiment (cell preparation, immunoblotting, and NMR- spectroscopy). There were so many materials I needed for the western blot that I had an entire slide dedicated for just the materials. I then had another slide just for the procedure of my western blot. Writing about the cell preparation and the NMR-Spectroscopy were easier because there were fewer steps and fewer materials, so I was able to fit everything onto one slide each. I also provided an introduction to my topic because I didn't define certain terms in my last presentation, and I was docked points for that. In order to make sure that everyone is understanding what I'm saying I summarized the background information one would need to understand my project. I spoke briefly on anabolic metabolism and biosynthesis, choline metabolism and GDPD-6, as well as glycerophosphocholine. I then launched into my questions and variables. In the variables section, I realized I wrote "control" where "constant" should be, but I covered it while presenting by saying "My controls and constants are...". I don't think anybody realized it though. I then spoke about the materials and methods used in each phase of my experiment. Finally, I presented my rationale. My presentation went well overall. It wasn't perfect, but it wasn't terrible. I was nervous at first but as soon as I started speaking, I knew what I was talking about. I was able to list the materials section for the western blot from pretty much memory. There were times that I stuttered or paused because I forgot the word I wanted to used, but I did better than before. I also became nervous again close to the end, but I managed to push through. I went over time however. I was only supposed to speak for 6-8 minutes, but spoke for 9 minutes 44 seconds. I believe it was just because I had a lot of materials I needed. In the final presentation, which can only be about 8 minutes I believe, I can shorten the time I spent on the methods section. I plan to generalize the materials list some more just to shorten it. I have to start working on everything, since we only have one more week before finals. Tags: Presentation, Reflection, Forward, Projects, Independent
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AuthorShubhangy Raghavan Archives
January 2019
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